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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which results in chromosome instability, and so may perhaps add to tumorigenesis. This protein was uncovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical part in advertising and marketing cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been explained. Two extra splice variants were being reported but detailed nucleotide sequence facts just isn't nonetheless offered.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide generally has the epitope recognized because of the antibody.
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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to make sure the very best benefits are...
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This guidebook outlines even more probable optimization of mobile and immunoassay parameters to make certain the absolute best final results are...
Antibodies certain to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is precise.
Focus on Information and facts Cyclin E belongs for the extremely conserved cyclin relatives, whose users are characterized by a spectacular periodicity in protein abundance throughout the mobile cycle. Cyclins functionality as regulators of CDK kinases. Various cyclins show distinct expression and degradation styles which lead to your temporal coordination of each mitotic celebration. Cyclin E varieties a complex with and capabilities as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress by means of S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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